Fetal ventral mesencephalon of human and rat origin maintained in vitro and transplanted to 6-hydroxydopamine-lesioned rats gives rise to grafts rich in dopaminergic neurons
Identifieur interne : 002176 ( Main/Exploration ); précédent : 002175; suivant : 002177Fetal ventral mesencephalon of human and rat origin maintained in vitro and transplanted to 6-hydroxydopamine-lesioned rats gives rise to grafts rich in dopaminergic neurons
Auteurs : Christian Spenger [Suisse] ; K. Haque [Royaume-Uni] ; Lorenz Studer [Suisse] ; Ljudmila Evtouchenko [Suisse] ; Bendicht Wagner [Suisse] ; Beatrice Bühler [Suisse] ; Urban Lendahl [Suède] ; B. Dunnett [Royaume-Uni] ; W. Seiler [Suisse]Source :
- Experimental Brain Research [ 0014-4819 ] ; 1996-11-01.
Abstract
Abstract: Free-floating roller tube cultures of human fetal (embryonic age 6–10 weeks post-conception) and rat fetal (embryonic day 13) ventral mesencephalon were prepared. After 7–15 days in vitro, the mesencephalic tissue cultures were transplanted into the striatum of adult rats that had received unilateral injections of 6-hydroxydopamine into the nigrostriatal bundle 3–5 weeks prior to transplantation. Graft survival was assessed in tyrosine hydroxylase (TH)-immunostained serial sections of the grafted brains up to post-transplantation week 4 for the human fetal xenografts and post-transplantation week 11 for the rat fetal allografts. d-amphetamine-induced rotation was monitored up to 10 weeks after transplantation in the allografted animals and compared with that of lesioned-only control animals. All transplanted animals showed large, viable grafts containing TH-immunoreactive (ir) neurons. The density of TH-ir neurons in the human fetal xenografts and in rat fetal allografts was similar. A significant amelioration of the amphetamine-induced rotation was observed in the animals that received cultured tissue allografts. These results promote the feasibility of in vitro maintenance of fetal human and rat nigral tissue prior to transplantation using the free-floating roller tube technique.
Url:
DOI: 10.1007/BF00227177
Affiliations:
- Royaume-Uni, Suisse, Suède
- Angleterre, Angleterre de l'Est, Canton de Berne, Svealand
- Berne, Cambridge, Stockholm
- Université de Berne, Université de Cambridge
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After 7–15 days in vitro, the mesencephalic tissue cultures were transplanted into the striatum of adult rats that had received unilateral injections of 6-hydroxydopamine into the nigrostriatal bundle 3–5 weeks prior to transplantation. Graft survival was assessed in tyrosine hydroxylase (TH)-immunostained serial sections of the grafted brains up to post-transplantation week 4 for the human fetal xenografts and post-transplantation week 11 for the rat fetal allografts. d-amphetamine-induced rotation was monitored up to 10 weeks after transplantation in the allografted animals and compared with that of lesioned-only control animals. All transplanted animals showed large, viable grafts containing TH-immunoreactive (ir) neurons. The density of TH-ir neurons in the human fetal xenografts and in rat fetal allografts was similar. A significant amelioration of the amphetamine-induced rotation was observed in the animals that received cultured tissue allografts. These results promote the feasibility of in vitro maintenance of fetal human and rat nigral tissue prior to transplantation using the free-floating roller tube technique.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li><li>Suisse</li><li>Suède</li></country><region><li>Angleterre</li><li>Angleterre de l'Est</li><li>Canton de Berne</li><li>Svealand</li></region><settlement><li>Berne</li><li>Cambridge</li><li>Stockholm</li></settlement><orgName><li>Université de Berne</li><li>Université de Cambridge</li></orgName></list><tree><country name="Suisse"><region name="Canton de Berne"><name sortKey="Spenger, Christian" sort="Spenger, Christian" uniqKey="Spenger C" first="Christian" last="Spenger">Christian Spenger</name></region><name sortKey="Buhler, Beatrice" sort="Buhler, Beatrice" uniqKey="Buhler B" first="Beatrice" last="Bühler">Beatrice Bühler</name><name sortKey="Evtouchenko, Ljudmila" sort="Evtouchenko, Ljudmila" uniqKey="Evtouchenko L" first="Ljudmila" last="Evtouchenko">Ljudmila Evtouchenko</name><name sortKey="Seiler, W" sort="Seiler, W" uniqKey="Seiler W" first="W." last="Seiler">W. Seiler</name><name sortKey="Studer, Lorenz" sort="Studer, Lorenz" uniqKey="Studer L" first="Lorenz" last="Studer">Lorenz Studer</name><name sortKey="Wagner, Bendicht" sort="Wagner, Bendicht" uniqKey="Wagner B" first="Bendicht" last="Wagner">Bendicht Wagner</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Haque, K" sort="Haque, K" uniqKey="Haque K" first="K." last="Haque">K. Haque</name></region><name sortKey="Dunnett, B" sort="Dunnett, B" uniqKey="Dunnett B" first="B." last="Dunnett">B. Dunnett</name></country><country name="Suède"><region name="Svealand"><name sortKey="Lendahl, Urban" sort="Lendahl, Urban" uniqKey="Lendahl U" first="Urban" last="Lendahl">Urban Lendahl</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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